Adaptive Optics

Adaptive Optics for Fluorescence Microscopy

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Introduction

Single molecule microscopy is a light microscopy technique that enables the detection of individual molecules, such as proteins, in a cellular context. The performance of this imaging modality can be enhanced by taking advantage of adaptive optics (AO). An AO system can actively correct the aberrations in the wavefront of light. The corrected wavefront in turn leads to enhanced performance of the acquisition system in terms of image sharpness and contrast. An AO system is typically comprised of two important components (see image above); one for sensing the wavefront and the other for correcting the wavefront. The former component is typically known as a wavefront sensor (WFS) and the latter is often referred to as a deformable mirror (DM), or an active mirror.

The optical test setup is shown below

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Software

  • Software prototyped in MATLAB (Optimization Toolbox, Statistics and Machine Learning Toolbox, Image Processing Toolbox)
  • Software developed in C++ (Visual Studio 2013)
  • Image processing using OpenCV
  • Based on object-oriented programming (has a graphical user interface)

The developed software for controling the adaptive optics.

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